Fluorescence-based microtiter plate assay for glutamate–cysteine ligase activity
نویسندگان
چکیده
منابع مشابه
Nonradioactive fluorescence microtiter plate assay monitoring aptamer selections.
Benchmarks Since its discovery in 1990, aptamer technology (1,2) has emerged as a novel and increasingly important approach to evolve specifically binding nucleic acid molecules (3). As aptamers can be developed to a vast range of target molecules, their potential application is very diverse. They can be used as molecular recognition elements in analytical systems for detection, separation, or ...
متن کاملMicrotiter plate immunoenzymometric assay for estrogen receptor.
The estrogen receptor (ER) status of breast cancer is used both as a prognostic factor and as a predictor of response to endocrine therapy. An immunoenzymometric assay for ER was developed on 96-well microtiter plates (EIA96). This technique involves two monoclonal antibodies directed against different epitopes in the B domain of ER. The two-step protocol (16-18 h and 3 h at 4 degrees C) requir...
متن کاملA Microtiter Plate-Based Assay for Inhibitors of Penicillin-Binding
PBP2a, the molecular determinant for high level β-lactam resistance in 19 Methicillin-resistant Staphylococcus aureus (MRSA), is intrinsically resistant to most β20 lactam antibiotics. The development and characterization of new inhibitors targeting 21 PBP2a would benefit from an effective and convenient assay for inhibitor binding. This 22 study was directed towards the development of a fluore...
متن کاملThe spheroplast lysis assay for yeast in microtiter plate format.
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for...
متن کاملA microtiter plate assay for the determination of the synthetic activity of protease.
Proteases have several industrial applications due to their hydrolytic activity [1] and have attracted considerable interest due to their ability to synthesize peptides in organic media [2,3]. Protease-catalyzed peptide synthesis occurs either by a thermodynamically controlled process (condensation of the acid and amine groups) or by a kinetically controlled process (aminolysis of an ester). In...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2003
ISSN: 0003-2697
DOI: 10.1016/s0003-2697(03)00143-x